Anti-Leishmanial Activity In Vitro and In Vivo of Allicin and Allicin Cream Using Leishmania major Sub-strain Zymowme LON4 and Balb-c MiceReport as inadecuate




Anti-Leishmanial Activity In Vitro and In Vivo of Allicin and Allicin Cream Using Leishmania major Sub-strain Zymowme LON4 and Balb-c Mice - Download this document for free, or read online. Document in PDF available to download.

Background

Leishmania is a unicellular protozoan parasite that produces several human diseases, ranging from localized self-healing cutaneous lesions to deadly visceral infections.

Objective

The effect of allicin on the growth of Leishmania major L. major promastigotes was evaluated under in vitro conditions. Moreover, the efficacy of a topical allicin cream was examined in BALB-c Bagg albino, laboratory-bred strain of the House Mouse mice with cutaneous leishmanial lesions compared to the currently used drug, sodiumstibogluconate pentostam.

Methods

Cytotoxiciy and promastigote proliferation were measured. Different concentrations 50, 100, 150, and 200 μM of liquid allicin were tested on L. major promastigotes twice: after 24 and 48 hours using an MTT colorimetric assay. In the in vivo condition, the efficacies of allicin cream and liquid allicin at two concentrations 0.15 μM-mouse and 0.30 μM-mouse were evaluated. Serum factors of the control and treated groups were tested to evaluate the toxic effects of allicin on the liver and kidney.

Results

Allicin at a concentration of 50 μM inhibited the growth of Leishmania promastigotes. Topical application of allicin cream reduced lesion sizes in mice. No significant differences in biochemical analysis were observed between the control and treated groups.

Conclusions

Allicin has antileishmanial effects under in vitro and in vivo conditions and may be used in clinical applications.



Author: Dina M. Metwally , Ebtesam M. Al-Olayan, Manal F. El-Khadragy, Badriah Alkathiri

Source: http://plos.srce.hr/



DOWNLOAD PDF




Related documents