Establishment of an Immortalized Skin Keratinocyte Cell Line Derived from the Animal Model Mastomys couchaReport as inadecuate




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In the present report we describe the establishment of a spontaneous immortalized skin keratinocyte cell line derived from the skin of the multimammate rodent Mastomys coucha. These animals are used in preclinical studies for a variety of human diseases such as infections with nematodes, bacteria and papillomaviruses, especially regarding cutaneous manifestations such as non-melanoma skin cancer. Here we characterize the cells in terms of their origin and cytogenetic features. Searching for genomic signatures, a spontaneous mutation in the splicing donor sequence of Trp53 G to A transition at the first position of intron 7 could be detected. This point mutation leads to alternative splicing and to a premature stop codon, resulting in a truncated and, in turn, undetectable form of p53, probably contributing to the process of immortalization. Mastomys coucha-derived skin keratinocytes can be used as an in vitro system to investigate molecular and immunological aspects of infectious agent interactions with their host cells.



Author: Daniel Hasche, Sonja Stephan, Larissa Savelyeva, Frank Westermann, Frank Rösl , Sabrina E. Vinzón

Source: http://plos.srce.hr/



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