The Effects of Paleoclimatic Events on Mediterranean Trout: Preliminary Evidences from Ancient DNAReport as inadecuate




The Effects of Paleoclimatic Events on Mediterranean Trout: Preliminary Evidences from Ancient DNA - Download this document for free, or read online. Document in PDF available to download.

In this pilot study for the first time, ancient DNA has been extracted from bone remains of Salmo trutta. These samples were from a stratigraphic succession located in a coastal cave of Calabria southern Italy inhabited by humans from upper Palaeolithic to historical times. Seven pairs of primers were used to PCR-amplify and sequence from 128 to 410 bp of the mtDNA control region of eleven samples. Three haplotypes were observed: two ADcs-1 and MEcs-1 already described in rivers from the Italian peninsula; one ATcs-33 belonging to the southern Atlantic clade of the AT Salmo trutta mtDNA lineage sensu Bernatchez. The prehistoric occurrence of this latter haplotype in the water courses of the Italian peninsula has been detected for the first time in this study. Finally, we observed a correspondence between frequency of trout remains and variation in haplotype diversity that we related with ecological and demographic changes resulting from a period of rapid cooling known as the Younger Dryas.



Author: Andrea Splendiani , Tatiana Fioravanti , Massimo Giovannotti, Alessandra Negri, Paolo Ruggeri, Luigi Olivieri, Paola Nisi Cerioni

Source: http://plos.srce.hr/



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