Saturated Fatty Acid-Induced Cytotoxicity in Liver Cells Does Not Involve Phosphatase and Tensin Homologue Deleted on Chromosome 10Report as inadecuate




Saturated Fatty Acid-Induced Cytotoxicity in Liver Cells Does Not Involve Phosphatase and Tensin Homologue Deleted on Chromosome 10 - Download this document for free, or read online. Document in PDF available to download.

Journal of Nutrition and MetabolismVolume 2013 2013, Article ID 514206, 8 pages

Research Article

Department of Food Science and Human Nutrition, Colorado State University, 234 Gifford, Fort Collins, CO 80523-1571, USA

Department of Biomedical Sciences, Colorado State University, Fort Collins, CO 80523, USA

Received 5 December 2012; Revised 11 March 2013; Accepted 25 March 2013

Academic Editor: Peter M. Clifton

Copyright © 2013 Dong Wang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Liver specific deletion of the tumor suppressor phosphatase and tensin homologue deleted on chromosome 10 PTEN induces steatosis and hypersensitivity to insulin. Saturated fatty acids, which induce endoplasmic reticulum stress and cell death, appear to increase PTEN, whereas unsaturated fatty acids which do not induce endoplasmic reticulum stress or cell death reduce this protein. In the present study, the role of PTEN in saturated fatty acid-induced cytotoxicity was examined in H4IIE and HepG2 liver cells. Palmitate and stearate increased the expression of PTEN, whereas the unsaturated fatty acids, oleate and linoleate, reduced PTEN expression in both cell types. SiRNA-mediated knockdown of PTEN did not increase liver cell triglyceride stores or reduce palmitate- or stearate-mediated ER stress or apoptosis. These results suggest that PTEN does not play a significant role in saturated fatty acid-induced cytotoxicity in these liver cell models and in the absence of insulin.





Author: Dong Wang, Yuren Wei, Melinda Frye, Christopher L. Gentile, and Michael J. Pagliassotti

Source: https://www.hindawi.com/



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