Dominant-Negative Effect of a Missense Variant in the TASK-2 KCNK5 K Channel Associated with Balkan Endemic NephropathyReport as inadecuate




Dominant-Negative Effect of a Missense Variant in the TASK-2 KCNK5 K Channel Associated with Balkan Endemic Nephropathy - Download this document for free, or read online. Document in PDF available to download.

TASK-2, a member of the Two-Pore Domain K2P subfamily of K+ channels, is encoded by the KCNK5 gene. The channel is expressed primarily in renal epithelial tissues and a potentially deleterious missense variant in KCNK5 has recently been shown to be prevalent amongst patients predisposed to the development of Balkan Endemic Nephropathy BEN, a chronic tubulointerstitial renal disease of unknown etiology. In this study we show that this variant T108P results in a complete loss of channel function and is associated with a major reduction in TASK-2 channel subunits at the cell surface. Furthermore, these mutant subunits have a suppressive or ‘dominant-negative’ effect on channel function when coexpressed with wild-type subunits. This missense variant is located at the extracellular surface of the M2 transmembrane helix and by using a combination of structural modelling and further functional analysis we also show that this highly-conserved threonine residue is critical for the correct function of other K2P channels. These results therefore provide further structural and functional insights into the possible pathophysiological effects of this missense variant in TASK-2.



Author: Alan P. Reed , Giovanna Bucci , Firdaus Abd-Wahab, Stephen J. Tucker

Source: http://plos.srce.hr/



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