Upregulation of Tumor Necrosis Factor-α-Induced Protein 8-Like 2 mRNA Is Negatively Correlated with Serum Concentrations of Tumor Necrosis Factor-α and Interleukin 6 in Type 2 Diabetes MellitusReport as inadecuate




Upregulation of Tumor Necrosis Factor-α-Induced Protein 8-Like 2 mRNA Is Negatively Correlated with Serum Concentrations of Tumor Necrosis Factor-α and Interleukin 6 in Type 2 Diabetes Mellitus - Download this document for free, or read online. Document in PDF available to download.

Journal of Diabetes Research - Volume 2017 2017, Article ID 4802319, 10 pages - https:-doi.org-10.1155-2017-4802319

Research Article

Central of Translation Medicine, Zibo Central Hospital Affiliated to Shandong University, Zibo 255036, China

Department of Pathology, Zibo Central Hospital Affiliated to Shandong University, Zibo 255036, China

Department of Central Laboratory, Zibo Central Hospital Affiliated to Shandong University, Zibo 255036, China

Correspondence should be addressed to Xinxin Xiang and Tao Li

Received 2 December 2016; Revised 4 March 2017; Accepted 4 April 2017; Published 24 May 2017

Academic Editor: Lucy Marzban

Copyright © 2017 Yongliang Liu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background. Tumor necrosis factor-α-induced protein 8-like 2 TIPE2 or TNFAIP8L2 is a negative regulator of natural and adaptive immunity. The role of TIPE2 in type 2 diabetes mellitus T2DM remains unknown, although TIPE2 plays key roles in preserving inflammatory homeostasis. Methods. TIPE2 expression was measured by Western blotting and real-time polymerase chain reaction RT-PCR in peripheral blood mononuclear cells PBMCs isolated from T2DM patients and healthy controls, and tumor necrosis factor-α TNF-α, high-sensitivity C-reactive protein hsCRP, interleukin 6 IL-6, and other related biometabolic parameters were detected using a nephelometer or by ELISA. Differentiated THP-1 cells were exposed to siTIPE2 and TIPE2 adenovirus. Results. TIPE2 was significantly increased in PBMCs from T2DM patients compared with those from healthy controls and was negatively correlated with serum TNF-α, IL-6, and hsCRP concentrations but positively correlated with HbA1c and LDL-C in T2DM patients. High glucose treatment 50 mmol-L can upregulate the expression of TIPE2 and cytokine secretion in differentiated THP-1 cells. siTIPE2 infection exacerbated the increased TNF-α and IL-6 concentrations in differentiated THP-1 cells under high glucose conditions 50 mmol-L, while infection with TIPE2 adenovirus reversed the increased TNF-α concentration. Conclusions. The present study indicates that TIPE2 may participate in T2DM by regulating TNF-α production.





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Source: https://www.hindawi.com/



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