P2X7 Gene Polymorphisms and Risk Assessment for Pulmonary Tuberculosis in Asian IndiansReport as inadecuate




P2X7 Gene Polymorphisms and Risk Assessment for Pulmonary Tuberculosis in Asian Indians - Download this document for free, or read online. Document in PDF available to download.

Disease Markers - Volume 28 2010, Issue 1, Pages 43-48



Department of Genetics, Bhagwan Mahavir Medical Research Centre, 10-1-1, A.C. Guards, Hyderabad, 500004, India

Department of Immunology, Bhagwan Mahavir Medical Research Centre, 10-1-1, A.C. Guards, Hyderabad, 500004, India

Blue Peter Research Centre, Cherlapally, Hyderabad, 501301, India

Department of Genetics & Molecular Medicine, Kamineni Hospitals. L.B. Nagar, Hyderabad, 500068, India

Department of Pulmonology, Kamineni Hospitals. L.B. Nagar, Hyderabad, 500068, India

Received 12 February 2010; Accepted 12 February 2010

Copyright © 2010 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Objective: Pulmonary tuberculosis PTB is a leading cause of morbidity and mortality. Macrophages play an important role in the immunopathogenesis of tuberculosis. Extracellular ATP induces macrophage bactericidal activity through activation of the purinergic P2X7 receptor. This case- control study assesses the association of −762 T-C, 1513A-C and 1729T-A P2X7 polymorphisms in patients with PTB and healthy controls to establish association if any with risk of developing the disease.

Materials and methods: The genotyping for P2X7 was carried out using PCR and RFLP analysis in 256 individuals, which included 156 active PTB patients and 100 age and sex, matched healthy volunteers with no clinical symptoms or family history of PTB as controls.

Results: A chi square test showed a significant difference between the PTB patient and controls for −762 C allele; p=0.0051 OR 1.6972, CI 95% 1.1839 to 2.4332 and1729 T allele was found to be positively associated with the PTB; p < 0.0005 OR-2.4623, CI 95% 1.6376 to 3.7022. 1513A-C polymorphism did not show any significant difference between the two groups.

Significance: The study revealed a significant association of P2X7-762C allele and P2X7 1729T allele receptor polymorphisms with PTB in Asian Indian population. The use of these alleles as biomarkers for identifying individuals at high risk of developing TB needs to be ascertained.





Author: Venkatasubramanian Sambasivan, Kolluri Janaki Rama Murthy, Ravindra Reddy, Valluri Vijayalakshimi, and Qurratulain Hasan

Source: https://www.hindawi.com/



DOWNLOAD PDF




Related documents