Crystal Structure of Human Myotubularin-Related Protein 1 Provides Insight into the Structural Basis of Substrate SpecificityReport as inadecuate




Crystal Structure of Human Myotubularin-Related Protein 1 Provides Insight into the Structural Basis of Substrate Specificity - Download this document for free, or read online. Document in PDF available to download.

Myotubularin-related protein 1 MTMR1 is a phosphatase that belongs to the tyrosine-dual-specificity phosphatase superfamily. MTMR1 has been shown to use phosphatidylinositol 3-monophosphate PI3P and-or phosphatidylinositol 3,5-bisphosphate PI3,5P2 as substrates. Here, we determined the crystal structure of human MTMR1. The refined model consists of the Pleckstrin homology PH-GRAM and phosphatase PTP domains. The overall structure was highly similar to the previously reported MTMR2 structure. Interestingly, two phosphate molecules were coordinated by strictly conserved residues located in the CX5R motif of the active site. Additionally, our biochemical studies confirmed the substrate specificity of MTMR1 for PI3P and PI3,5P2 over other phosphatidylinositol phosphates. Our structural and enzymatic analyses provide insight into the catalytic mechanism and biochemical properties of MTMR1.



Author: Seoung Min Bong, Kka-bi Son, Seung-Won Yang, Jae-Won Park, Jea-Won Cho, Kyung-Tae Kim, Hackyoung Kim, Seung Jun Kim, Young Jun Ki

Source: http://plos.srce.hr/



DOWNLOAD PDF




Related documents