Human Embryonic Stem Cell Lines with Lesions in FOXP3 and NF1Report as inadecuate




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Human embryonic stem cells hESCs are derived from the inner cell mass ICM of blastocyst staged embryos. Spare blastocyst staged embryos were obtained by in vitro fertilization IVF and donated for research purposes. hESCs carrying specific mutations can be used as a powerful cell system in modeling human genetic disorders. We obtained preimplantation genetic diagnosed PGD blastocyst staged embryos with genetic mutations that cause human disorders and derived hESCs from these embryos. We applied laser assisted micromanipulation to isolate the inner cell mass from the blastocysts and plated the ICM onto the mouse embryonic fibroblast cells. Two hESC lines with lesions in FOXP3 and NF1 were established. Both lines maintain a typical undifferentiated hESCs phenotype and present a normal karyotype. The two lines express a panel of pluripotency markers and have the potential to differentiate to the three germ layers in vitro and in vivo. The hESC lines with lesions in FOXP3 and NF1 are available for the scientific community and may serve as an important resource for research into these disease states.



Author: Hui Zhu, Barry Behr, Vikrant V. Reddy, Mark Hughes, Yuqiong Pan, Julie Baker

Source: http://plos.srce.hr/



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