Effect of Aminated Mesoporous Bioactive Glass Nanoparticles on the Differentiation of Dental Pulp Stem CellsReport as inadecuate

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Mesoporous bioactive nanoparticles MBNs have been developed as promising additives to various types of bone or dentin regenerative material. However, biofunctionality of MBNs as dentin regenerative additive to dental materials have rarely been studied. We investigated the uptake efficiency of MBNs-NH2 with their endocytosis pathway and the role of MBNs-NH2 in odontogenic differentiation to clarify inherent biofunctionality. MBNs were fabricated by sol-gel synthesis, and 3% APTES was used to aminate these nanoparticles MBNs-NH2 to reverse their charge from negative to positive. To characterize the MBNs-NH2, TEM, XRD, FTIR, zetaξ-potential measurements, and Brunauer–Emmett–Teller analysis were performed. After primary cultured rat dental pulp stem cells rDPSCs were incubated with various concentrations of MBNs-NH2, stem cell viability 24 hours with or without differentiated media, internalization of MBNs-NH2 in rDPSCs ~4 hours via specific endocytosis pathway, intra or extracellular ion concentration and odontoblastic differentiation ~28 days were investigated. Incubation with up to 50 μg-mL of MBNs-NH2 had no effect on rDPSCs viability with differentiated media p>0.05. The internalization of MBNs-NH2 in rDPSCs was determined about 92% after 4 hours of incubation. Uptake was significantly decreased with ATP depletion and after 1 hour of pre-treatment with the inhibitor of macropinocytosis p<0.05. There was significant increase of intracellular Ca and Si ion concentration in MBNs-NH2 treated cells compared to no-treated counterpart p<0.05. The expression of odontogenic-related genes BSP, COL1A, DMP-1, DSPP, and OCN and the capacity for biomineralization based on alkaline phosphatase activity and alizarin red staining were significantly upregulated with MBNs-NH2. These results indicate that MBNs-NH2 induce odontogenic differentiation of rDPSCs and may serve as a potential dentin regenerative additive to dental material for promoting odontoblast differentiation.

Author: Jung-Hwan Lee , Min-Sil Kang, Chinmaya Mahapatra, Hae-Won Kim

Source: http://plos.srce.hr/


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