Transcriptome Analysis Provides Insights into the Mechanisms Underlying Wheat Plant Resistance to Stripe Rust at the Adult Plant StageReport as inadecuate




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Stripe rust or yellow rust, which is caused by Puccinia striiformis f. sp. tritici Pst, is one of the most devastating wheat diseases worldwide. The wheat cultivar Xingzi 9104 XZ is an elite wheat germplasm that possesses adult plant resistance APR, which is non–race-specific and durable. Thus, to better understand the mechanism underlying APR, we performed transcriptome sequencing of wheat seedlings and adult plants without Pst infection, and a total of 157,689 unigenes were obtained as a reference. In total, 2,666, 783 and 2,587 differentially expressed genes DEGs were found to be up- or down-regulated after Pst infection at 24, 48 and 120 hours post-inoculation hpi, respectively, based on a comparison of Pst- and mock-infected plants. Among these unigenes, the temporal pattern of the up-regulated unigenes exhibited transient expression patterns during Pst infection, as determined through a Gene Ontology GO enrichment analysis. In addition, a Kyoto Encyclopedia of Genes and Genomes KEGG pathway analysis showed that many biological processes, including phenylpropanoid biosynthesis, reactive oxygen species, photosynthesis and thiamine metabolism, which mainly control the mechanisms of lignification, reactive oxygen species and sugar, respectively, are involved in APR. In particular, the continuous accumulation of reactive oxygen species may potentially contribute to the ability of the adult plant to inhibit fungal growth and development. To validate the bioinformatics results, 6 candidate genes were selected for further functional identification using the virus-induced gene silencing VIGS system, and 4 candidate genes likely contribute to plant resistance against Pst infection. Our study provides new information concerning the transcriptional changes that occur during the Pst-wheat interaction at the adult stage and will help further our understanding of the detailed mechanisms underlying APR to Pst.



Author: Yingbin Hao, Ting Wang, Kang Wang, Xiaojie Wang , Yanping Fu, Lili Huang, Zhensheng Kang

Source: http://plos.srce.hr/



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