Using Gelatin Nanoparticle Mediated Intranasal Delivery of Neuropeptide Substance P to Enhance Neuro-Recovery in Hemiparkinsonian RatsReport as inadecuate




Using Gelatin Nanoparticle Mediated Intranasal Delivery of Neuropeptide Substance P to Enhance Neuro-Recovery in Hemiparkinsonian Rats - Download this document for free, or read online. Document in PDF available to download.

Purpose

Intranasal administration of phospholipid-based gelatin nanoparticles GNP was prepared to investigate the neuro-recovery effects of neuropeptide Substance P SP on hemiparkinsonian rats.

Methods

The SP-loaded gelatin nanoparticles SP-GNP were prepared by a water-in-water emulsion method and possessed high stability, encapsulating efficiency and loading capacity. PC-12 cells were used to examine the growth enhancement of SP-GNP in vitro by MTT assays and flow cytometry FCM. The therapeutic effects of SP-GNP on 6-hydroxydopamine 6-OHDA induced hemiparkinsonian rats were assessed by quantifying rotational behavior and the levels of tyrosine hydroxylase TH, phosphorylated c-Jun protein p-c-Jun and Caspase-3 Cas-3 expressed in substantia nigra SN region of hemiparkinsonian rats.

Results

PC-12 cells under SP-GNP treatment showed better cell viability and lower degree of apoptosis than those under SP solution treatment. Hemiparkinsonian rats under intranasal SP-GNP administration demonstrated better behavioral improvement, higher level of TH in SN along with much lower extent of p-c-Jun and Cas-3 than those under intranasal SP solution administration and intravenous SP-GNP administration.

Conclusions

With the advantages of GNP and nose-to-brain pathway, SP can be effectively delivered into the damaged SN region and exhibit its neuro-recovery function through the inhibition on JNK pathway and dopaminergic neuron apoptosis.



Author: Ying-Zheng Zhao , Rong-Rong Jin , Wei Yang , Qi Xiang, Wen-Ze Yu, Qian Lin, Fu-Rong Tian, Kai-Li Mao, Chuan-Zhu Lv, Yi-Xiáng J.

Source: http://plos.srce.hr/



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