Diurnal variation in cholesterol 7α-hydroxylase activity is determined by the -203A>C polymorphism of the CYP7A1 geneReport as inadecuate




Diurnal variation in cholesterol 7α-hydroxylase activity is determined by the -203A>C polymorphism of the CYP7A1 gene - Download this document for free, or read online. Document in PDF available to download.

Croatian medical journal, Vol.57 No.2 April 2016. -

Aim To determine whether the promoter polymorphism

-203A>C of cholesterol-7α-hydroxylase encoding gene CYP7A1

affects diurnal variation in CYP7A1 enzyme activity.

Methods The study included 16 healthy male volunteers –

8 homozygous for -203A and 8 homozygous for the -203C

allele of CYP7A1. Three 15-hour examinations from 7am

to 10pm were carried out for each of the participants: after

one-day treatment with cholestyramine; after one-day

treatment with chenodeoxycholic acid CDCA; and a control

examination without any treatment. The plasma concentration

of 7α-hydroxy-4-cholesten-3-one C4, a marker

of CYP7A1 activity, was determined in all the experiments

at 90-min intervals.

Results CYP7A1 activity was up-regulated after treatment

with cholestyramine and suppressed after treatment

with CDCA. There were no differences between -203A and

-203C allele carriers in the response of enzyme activity to

both drugs. In the control experiment -203A allele carriers

displayed diurnal variation in enzyme activity, whereas CYP7A1

activity did not change in -203C allele carriers. These

results were confirmed by modeling the dynamics of C4

using polynomial regression.

Conclusion The promoter polymorphism of the CYP7A1

gene has a pronounced impact on diurnal variation in CYP7A1

activity.



Author: Miluše Vlachová - ; Institute for Clinical and Experimental Medicine, Prague Czech Republic Tereza Blahová - ; Institute for C

Source: http://hrcak.srce.hr/



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