The association of dimethylsulfoxide and model membranes studied by pulse-field gradient NMRReport as inadecuate




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Spectroscopy - Volume 18 2004, Issue 2, Pages 265-269



Department of Blood Research, Division of Military Casualty Research, Walter Reed Army Institute of Research, Silver Spring, MD, USA

Department of Medicinal Chemistry, Division of Experimental Therapeutics, Walter Reed Army Institute of Research, Silver Spring, MD, USA

Department of Blood Research, Walter Reed Army Institute of Research, Silver Spring, MD 20910, USA



Copyright © 2004 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The use of dimethylsulfoxide DMSO as a cryoprotectant to reduce cellular injury during freezing is well known, however the intermolecular interactions between this amphiphilic molecule and biological membranes that form the basis of this protection are unknown. DMSO–dipalmitoylphosphatidylcholine DPPC vesicle interactions were investigated in pulsed-field gradient NMR PFGNMR experiments and spectra analysis allowed for the determination of self-diffusion coefficients for each species present. The mole fraction of DMSO associated with the DPPC vesicles was then calculated from the diffusion coefficients: the mole fraction increased from 14% to 42% as the membrane was heated from below to above the main phase transition temperature.





Author: Anthony Kennedy, Carla J. Long, Peter J. Hmel, Rickey Hicks, and Thomas J. Reid

Source: https://www.hindawi.com/



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