Genetic Evidence for the Role of the Vacuole in Supplying Secretory Organelles with Ca2 in Hansenula polymorphaReport as inadecuate




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Processes taking place in the secretory organelles require Ca2+ and Mn2+, which in yeast are supplied by the Pmr1 ion pump. Here we observed that in the yeast Hansenula polymorpha Ca2+ deficiency in the secretory pathway caused by Pmr1 inactivation is exacerbated by i the ret1-27 mutation affecting COPI-mediated vesicular transport, ii inactivation of the vacuolar Ca2+ ATPase Pmc1 and iii inactivation of Vps35, which is a component of the retromer complex responsible for protein transport between the vacuole and secretory organelles. The ret1-27 mutation also exerted phenotypes indicating alterations in transport between the vacuole and secretory organelles. These data indicate that ret1-27, pmc1 and vps35 affect a previously unknown Pmr1-independent route of the Ca2+ delivery to the secretory pathway. We also observed that the vacuolar protein carboxypeptidase Y receives additional modifications of its glycoside chains if it escapes the Vps10-dependent sorting to the vacuole.



Author: Anastasia V. Fokina, Maria B. Chechenova, Azamat V. Karginov, Michael D. Ter-Avanesyan, Michael O. Agaphonov

Source: http://plos.srce.hr/



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