Two-Dimensional Differential Gel Electrophoresis to Identify Protein Biomarkers in Amniotic Fluid of Edwards Syndrome Trisomy 18 PregnanciesReport as inadecuate




Two-Dimensional Differential Gel Electrophoresis to Identify Protein Biomarkers in Amniotic Fluid of Edwards Syndrome Trisomy 18 Pregnancies - Download this document for free, or read online. Document in PDF available to download.

Background

Edwards syndrome ES is a severe chromosomal abnormality with a prevalence of about 0.8 in 10,000 infants born alive. The aims of this study were to identify candidate proteins associated with ES pregnancies from amniotic fluid supernatant AFS using proteomics, and to explore the role of biological networks in the pathophysiology of ES.

Methods

AFS from six second trimester pregnancies with ES fetuses and six normal cases were included in this study. Fluorescence-based two-dimensional difference gel electrophoresis 2D-DIGE and matrix-assisted laser desorption-ionization time-of-flight mass spectrometry MALDI-TOF-MS were used for comparative proteomic analysis. The identified proteins were further validated by Western blotting and the role of biological networks was analyzed.

Results

Twelve protein spots were differentially expressed by more than 1.5-fold in the AFS of the ES pregnancies. MALDI-TOF-MS identified one up-regulated protein: apolipoprotein A1 ApoA1, and four under-regulated proteins: vitamin D binding protein VDBP, alpha-1-antitrypsin A1AT, insulin-like growth factor-binding protein 1 IGFBP-1, and transthyretin TTR. Western blot and densitometric analysis of ApoA1, A1AT, IGFBP-1, and TTR confirmed the alteration of these proteins in the amniotic fluid samples. Biological network analysis revealed that the proteins of the ES AFS were involved mainly in lipid and hormone metabolism, immune response, and cardiovascular disease.

Conclusions

These five proteins may be involved in the pathogenesis of ES. Further studies are needed to explore.



Author: Te-Yao Hsu , Hao Lin, Hsuan-Ning Hung, Kuender D. Yang, Chia-Yu Ou, Ching-Chang Tsai, Hsin-Hsin Cheng, Su-Hai Chung, Bi-Hua Cheng

Source: http://plos.srce.hr/



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