Short Communication: Subtyping of Mycobacterium kansasii by PCR-Restriction Enzyme Analysis of the hsp65 GeneReport as inadecuate




Short Communication: Subtyping of Mycobacterium kansasii by PCR-Restriction Enzyme Analysis of the hsp65 Gene - Download this document for free, or read online. Document in PDF available to download.

BioMed Research InternationalVolume 2013 2013, Article ID 178725, 4 pages

Research Article

Department of Applied Microbiology, Institute of Microbiology, Faculty of Biology, University of Warsaw, I. Miecznikowa 1, 02-096 Warsaw, Poland

Department of Internal Medicine, Pneumonology, and Allergology, Medical University of Warsaw, Żwirki i Wigury 61, 02-091 Warsaw, Poland

Clinic of Internal Medicine, Pneumonology, and Allergology, Independent Public Central Clinical Hospital, S. Banacha 1A, 02-097 Warsaw, Poland

Received 8 November 2013; Accepted 9 December 2013

Academic Editor: Jarosław Dziadek

Copyright © 2013 Zofia Bakuła et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Mycobacterium kansasii is one of the most common causes of pulmonary disease resulting from nontuberculous mycobacteria NTM. It is also the most frequently isolated NTM species from clinical specimens in Poland. The aim of this study was to investigate the distribution of M. kansasii subtypes among patients suspected of having pulmonary NTM disease. Fifty clinical isolates of M. kansasii recovered from as many patients with suspected mycobacterial lung disease between 2000 and 2010 in Poland were genotyped by PCR-restriction enzyme analysis PCR-REA of partial hsp65 gene. Mycobacterium kansasii subtype I was the only genotype to be identified among the isolates, both disease-associated and non-disease-associated. Isolation of M. kansasii subtype I from clinical specimens may be indicative of infection but may also merely represent colonization.





Author: Zofia Bakuła, Aleksandra Safianowska, Magdalena Nowacka-Mazurek, Jacek Bielecki, and Tomasz Jagielski

Source: https://www.hindawi.com/



DOWNLOAD PDF




Related documents