The Mouse Solitary Odorant Receptor Gene Promoters as Models for the Study of Odorant Receptor Gene ChoiceReport as inadecuate




The Mouse Solitary Odorant Receptor Gene Promoters as Models for the Study of Odorant Receptor Gene Choice - Download this document for free, or read online. Document in PDF available to download.

Background

In vertebrates, several anatomical regions located within the nasal cavity mediate olfaction. Among these, the main olfactory epithelium detects most conventional odorants. Olfactory sensory neurons, provided with cilia exposed to the air, detect volatile chemicals via an extremely large family of seven-transmembrane chemoreceptors named odorant receptors. Their genes are expressed in a monogenic and monoallelic fashion: a single allele of a single odorant receptor gene is transcribed in a given mature neuron, through a still uncharacterized molecular mechanism known as odorant receptor gene choice.

Aim

Odorant receptor genes are typically arranged in genomic clusters, but a few are isolated we call them solitary from the others within a region broader than 1 Mb upstream and downstream with respect to their transcript-s coordinates. The study of clustered genes is problematic, because of redundancy and ambiguities in their regulatory elements: we propose to use the solitary genes as simplified models to understand odorant receptor gene choice.

Procedures

Here we define number and identity of the solitary genes in the mouse genome C57BL-6J, and assess the conservation of the solitary status in some mammalian orthologs. Furthermore, we locate their putative promoters, predict their homeodomain binding sites commonly present in the promoters of odorant receptor genes and compare candidate promoter sequences with those of wild-caught mice. We also provide expression data from histological sections.

Results

In the mouse genome there are eight intact solitary genes: Olfr19 M12, Olfr49, Olfr266, Olfr267, Olfr370, Olfr371, Olfr466, Olfr1402; five are conserved as solitary in rat. These genes are all expressed in the main olfactory epithelium of three-day-old mice. The C57BL-6J candidate promoter of Olfr370 has considerably varied compared to its wild-type counterpart. Within the putative promoter for Olfr266 a homeodomain binding site is predicted. As a whole, our findings favor Olfr266 as a model gene to investigate odorant receptor gene choice.



Author: Andrea Degl-Innocenti , Marta Parrilla , Bettina Harr , Meike Teschke

Source: http://plos.srce.hr/



DOWNLOAD PDF




Related documents