Proteomic Analysis of Silk Viability in Maize Inbred Lines and Their Corresponding HybridsReport as inadecuate




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A long period of silk viability is critical for a good seed setting rate in maize Zea mays L., especially for inbred lines and hybrids with a long interval between anthesis and silking. To explore the molecular mechanism of silk viability and its heterosis, three inbred lines with different silk viability characteristics Xun928, Lx9801, and Zong3 and their two hybrids Xun928×Zong3 and Lx9801×Zong3 were analyzed at different developmental stages by a proteomic method. The differentially accumulated proteins were identified by mass spectrometry and classified into metabolism, protein biosynthesis and folding, signal transduction and hormone homeostasis, stress and defense responses, and cellular processes. Proteins involved in nutrient methionine and energy ATP supply, which support the pollen tube growth in the silk, were important for silk viability and its heterosis. The additive and dominant effects at a single locus, as well as complex epistatic interactions at two or more loci in metabolic pathways, were the primary contributors for mid-parent heterosis of silk viability. Additionally, the proteins involved in the metabolism of anthocyanins, which indirectly negatively regulate local hormone accumulation, were also important for the mid-parent heterosis of silk viability. These results also might imply the developmental dependence of heterosis, because many of the differentially accumulated proteins made distinct contributions to the heterosis of silk viability at specific developmental stages.



Author: Zhihui Ma , Yongtian Qin , Yafei Wang, Xiaofeng Zhao, Fangfang Zhang, Jihua Tang, Zhiyuan Fu

Source: http://plos.srce.hr/



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