Structure and Stability of FlgD from the Pathogenic 26695 Strain of Helicobacter pyloriReport as inadecuate




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Croatica Chemica Acta, Vol.89 No.2 June 2016. -

Flagellin component D FlgD is a hook capping protein involved in the proper structural assembly of bacterial flagella but is not present in the mature flagella. We report here the crystal structure of the truncated form of FlgD from the strain 26695 of Helicobacter pylori

HpFlgD 26695 in the P2 space group at 2.8 Å resolution, along with data on protein stability. The structure includes only the central globular part of the protein composed of conserved Fn-III and tudor domains, while the N- and C-terminal regions are absent. This structure, together with the tetragonal crystal structure of HpFlgD 26695 and the monoclinic crystal structure of HpFlgD G27 reported previously, revealed a different mutual orientation of the two domains in the HpFlgD structures in respect to FlgD from Xanthomonas campestris and Pseudomonas aeruginosa. In addition, investigation of protein stability in solution showed higher protein stability then in other bacterial orthologues reported so far.

This work is licensed under a Creative Commons Attribution 4.0 International License.

Helicobacter pylori; strain 26695; FlgD; stability; monoclinic crystal structure



Author: Ivana Kekez - orcid.org-0000-0001-8690-5465 ; University of Zagreb, Faculty of Science, Department of Chemistry, Division of Gene

Source: http://hrcak.srce.hr/



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