Improving the Diagnosis of Bloodstream Infections: PCR Coupled with Mass SpectrometryReport as inadecuate




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BioMed Research International - Volume 2014 2014, Article ID 501214, 8 pages -

Review Article

Servei de Microbiologia, Fundació Institut d’Investigació en Ciències de la Salut Germans Trias i Pujol, Hospital Universitari Germans Trias i Pujol, Universitat Autònoma de Barcelona, 08916 Badalona, Spain

CIBER Enfermedades Respiratorias CIBERES, 07110 Bunyola, Spain

CIBER Epidemiología y Salud Pública CIBERESP, 08036 Barcelona, Spain

Received 27 December 2013; Accepted 27 February 2014; Published 9 April 2014

Academic Editor: Renu Bharadwaj

Copyright © 2014 Elena Jordana-Lluch et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The reference method for the diagnosis of bloodstream infections is blood culture followed by biochemical identification and antibiotic susceptibility testing of the isolated pathogen. This process requires 48 to 72 hours. The rapid administration of the most appropriate antimicrobial treatment is crucial for the survival of septic patients; therefore, a rapid method that enables diagnosis directly from analysis of a blood sample without culture is needed. A recently developed platform that couples broad-range PCR amplification of pathogen DNA with electrospray ionization mass spectrometry PCR-ESI-MS has the ability to identify virtually any microorganism from direct clinical specimens. To date, two clinical evaluations of the PCR-ESI-MS technology for the diagnosis of bloodstream infections from whole blood have been published. Here we discuss them and describe recent improvements that result in an enhanced sensitivity. Other commercially available assays for the molecular diagnosis of bloodstream infections from whole blood are also reviewed. The use of highly sensitive molecular diagnostic methods in combination with conventional procedures could substantially improve the management of septic patients.





Author: Elena Jordana-Lluch, Montserrat Giménez, M. Dolores Quesada, Vicente Ausina, and Elisa Martró

Source: https://www.hindawi.com/



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