Effects of Shen-Fu Injection on the Expression of T-Cell-Specific Transcription Factors T-bet-Gata-3 in Porcine Postresuscitation Lung InjuryReport as inadecuate




Effects of Shen-Fu Injection on the Expression of T-Cell-Specific Transcription Factors T-bet-Gata-3 in Porcine Postresuscitation Lung Injury - Download this document for free, or read online. Document in PDF available to download.

Evidence-Based Complementary and Alternative MedicineVolume 2013 2013, Article ID 464650, 8 pages

Research ArticleDepartment of Emergency Medicine, Beijing Chaoyang Hospital, Capital Medical University, Beijing 100020, China

Received 28 November 2012; Accepted 17 February 2013

Academic Editor: Roci­o De la Puerta

Copyright © 2013 Wei Gu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Shen-Fu injection SFI derived from the ancient traditional Chinese medicine. In this study, the effects of SFI on the expression of T-bet-GATA-3 and its potential mechanisms causing the shift of T cells from Th2 to Th1 on postresuscitation lung injury were examined in a porcine model of cardiac arrest. 30 pigs were randomly divided into SHAM and three return of spontaneous circulation ROSC groups per group; 24 pigs were subjected to 8 min of electrically induced cardiac arrest and 2 min of basic life support, which received central venous injection of Shen-Fu SFI, epinephrine EP or saline SA. After successful ROSC, 18 surviving pigs were sacrificed at 24 h after ROSC per group. The levels of serum and lung tissue interleukin IL-4 and interferon IFN-γ were measured by ELISA, and the protein and mRNA levels of GATA-3 and T-bet in the lung tissue were determined by western blotting and quantitative real-time polymerase chain reaction, respectively. Compared with the EP and SA groups, SFI treatment reduced the levels of IL-4 , increased levels of IFN-γ , and induced T-bet mRNA upregulation and GATA-3 mRNA downregulation . SFI attenuated lung injury and regulated lung immune disorders. Therefore, SFI could protect postresuscitation lung injury by modulating a Th1-Th2 imbalance.





Author: Wei Gu, ChunSheng Li, WenPeng Yin, XiaoMin Hou, and Da Zhang

Source: https://www.hindawi.com/



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