Lifetime estimation of moving vesicles in frequency-domain fluorescence lifetime imaging microscopyReport as inadecuate




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* Corresponding author 1 SERPICO - Space-timE RePresentation, Imaging and cellular dynamics of molecular COmplexes Inria Rennes – Bretagne Atlantique 2 PICT-IBiSA - BioImaging Cell and Tissue Core Facility 3 Mécanismes moléculaires du transport intracellulaire CDC - Compartimentation et dynamique cellulaires

Abstract : We propose a framework for correcting the effect of vesicles motion in frequency domain FLIM imaging. Estimation of movement and lifetime are decoupled and alternatively performed. Robust M-estimation is involved to improve the accuracy of our estimate. Our method has been evaluated with both simulated and real samples.

Keywords : FLIM frequency-domain phase modulation particle tracking template matching M-estimator





Author: Philippe Roudot - Charles Kervrann - François Waharte -

Source: https://hal.archives-ouvertes.fr/



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