In Vitro Assessment of Anthelmintic Activities of Rauwolfia vomitoria Apocynaceae Stem Bark and Roots against Parasitic Stages of Schistosoma mansoni and Cytotoxic StudyReport as inadecuate




In Vitro Assessment of Anthelmintic Activities of Rauwolfia vomitoria Apocynaceae Stem Bark and Roots against Parasitic Stages of Schistosoma mansoni and Cytotoxic Study - Download this document for free, or read online. Document in PDF available to download.

Journal of Parasitology Research - Volume 2017 2017, Article ID 2583969, 11 pages - https:-doi.org-10.1155-2017-2583969

Research Article

Laboratory for Tuberculosis Research and Pharmacology, Biotechnology Centre, Nkolbisson, University of Yaoundé 1, Yaoundé, Cameroon

Noguchi Memorial Institute for Medical Research NMIMR, College of Health Sciences, University of Ghana, PO Box LG581 Legon, Accra, Ghana

Centre for Plant Medicine Research CPMR, Akwapim, Mampong, Ghana

School of Pharmacy, College of Health Sciences, University of Ghana, Accra, Ghana

Correspondence should be addressed to Emmanuel Mouafo Tekwu

Received 15 December 2016; Accepted 9 February 2017; Published 28 February 2017

Academic Editor: Bernard Marchand

Copyright © 2017 Emmanuel Mouafo Tekwu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Schistosomiasis is a Neglected Tropical Diseases which can be prevented with mass deworming chemotherapy. The reliance on a single drug, praziquantel, is a motivation for the search of novel antischistosomal compounds. This study investigated the anthelmintic activity of the stem bark and roots of Rauwolfia vomitoria against two life stages of Schistosoma mansoni. Both plant parts were found to be active against cercariae and adult worms. Within 2 h of exposure all cercariae were killed at a concentration range of 62.5–1000 µg-mL and 250–1000 µg-mL of R. vomitoria stem bark and roots, respectively. The LC50 values determined for the stem bark after 1 and 2 h of exposure were 207.4 and 61.18 µg-mL, respectively. All adult worms exposed to the concentrations range of 250–1000 µg-mL for both plant parts died within 120 h of incubation. The cytotoxic effects against HepG2 and Chang liver cell assessed using MTT assay method indicated that both plant extracts which were inhibitory to the proliferation of cell lines with IC50 > 20 μg-mL appear to be safe. This report provides the first evidence of in vitro schistosomicidal potency of R. vomitoria with the stem bark being moderately, but relatively, more active and selective against schistosome parasites. This suggests the presence of promising medicinal constituents.





Author: Emmanuel Mouafo Tekwu, Kwabena Mante Bosompem, William Kofi Anyan, Regina Appiah-Opong, Kofi Baffour-Awuah Owusu, Mabel Delad

Source: https://www.hindawi.com/



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