GSTP1 Methylation and Protein Expression in Prostate Cancer: Diagnostic ImplicationsReport as inadecuate




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Disease Markers - Volume 2016 2016, Article ID 4358292, 6 pages -

Research Article

Biosciences Laboratory, Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori IRST IRCCS, Via P. Maroncelli 40, 47014 Meldola, Italy

University of Florence, 50121 Florence, Italy

Pathology Unit, Morgagni-Pierantoni Hospital, 47121 Forlì, Italy

Department of Urology, Morgagni-Pierantoni Hospital, 47121 Forlì, Italy

Department of Medical Oncology, Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori IRST IRCCS, Via P. Maroncelli 40, 47014 Meldola, Italy

Unit of Biostatistics and Clinical Trials, Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori IRST IRCCS, Via P. Maroncelli 40, 47014 Meldola, Italy

Received 17 February 2016; Revised 21 June 2016; Accepted 3 July 2016

Academic Editor: Carmen Jeronimo

Copyright © 2016 Filippo Martignano et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

GSTP1 belongs to the GSTs family, a group of enzymes involved in detoxification of exogenous substances and it also plays an important role in cell cycle regulation. Its dysregulation correlates with a large variety of tumors, in particular with prostate cancer. We investigated GSTP1 methylation status with methylation specific PCR MS-PCR in prostate cancer PCa and in benign tissue of 56 prostatectomies. We also performed immunohistochemistry IHC so as to correlate gene methylation with gene silencing. GSTP1 appears methylated in PCa and not in healthy tissue; IHC confirmed that methylation leads to protein underexpression . GSTP1 is highly expressed in basal cell layer and luminal cells in benign glands while in prostatic intraepithelial neoplasia PIN it stains only basal cell layer, whereas PCa glands are completely negative. We demonstrated that methylation leads to underexpression of GSTP1. The progressive loss of GSTP1 expression from healthy glands to PIN and to PCa glands underlines its involvement in early carcinogenesis.





Author: Filippo Martignano, Giorgia Gurioli, Samanta Salvi, Daniele Calistri, Matteo Costantini, Roberta Gunelli, Ugo De Giorgi, Fl

Source: https://www.hindawi.com/



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