Effect of Piper betle and Brucea javanica on the Differential Expression of Hyphal Wall Protein HWP1 in Non-Candida albicans Candida NCAC SpeciesReport as inadecuate




Effect of Piper betle and Brucea javanica on the Differential Expression of Hyphal Wall Protein HWP1 in Non-Candida albicans Candida NCAC Species - Download this document for free, or read online. Document in PDF available to download.

Evidence-Based Complementary and Alternative MedicineVolume 2013 2013, Article ID 397268, 6 pages

Research ArticleDepartment of Oral Biology, Faculty of Dentistry, University of Malaya, 50603 Kuala Lumpur, Malaysia

Received 1 April 2013; Accepted 28 May 2013

Academic Editor: Mark Moss

Copyright © 2013 Wan Himratul Aznita Wan Harun et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The study aimed to identify the HWP1 gene in non-Candida albicans Candida species and the differential expression of HWP1 following treatment with Piper betle and Brucea javanica aqueous extracts. All candidal suspensions were standardized to  cells-mL. The suspension was incubated overnight at 37 °C C. parapsilosis, 35°C. Candidal cells were treated with each respective extract at 1, 3, and 6 mg-mL for 24 h. The total RNA was extracted and reverse transcription-polymerase chain reaction was carried out with a specific primer of HWP1. HWP1 mRNAs were only detected in C. albicans, C. parapsilosis, and C. tropicalis. Exposing the cells to the aqueous extracts has affected the expression of HWP1 transcripts. C. albicans, C. parapsilosis, and C. tropicalis have demonstrated different intensity of mRNA. Compared to P. betle, B. javanica demonstrated a higher suppression on the transcript levels of HWP1 in all samples. HWP1 was not detected in C. albicans following the treatment of B. javanica at 1 mg-mL. In contrast, C. parapsilosis and C. tropicalis were shown to have HWP1 regulation. However, the expression levels were reduced upon the addition of higher concentration of B. javanica extract. P. betle and B. javanica have potential to be developed as oral health product.





Author: Wan Himratul Aznita Wan Harun, Nur Alyaa Jamil, Nor Hazwani Jamaludin, and Mohd-Al-Faisal Nordin

Source: https://www.hindawi.com/



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