The Increased Expression of Connexin and VEGF in Mouse Ovarian Tissue Vitrification by Follicle Stimulating HormoneReport as inadecuate

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BioMed Research International - Volume 2015 2015, Article ID 397264, 13 pages -

Research Article

Key Laboratory of Fertility Preservation and Maintenance of Ministry of Education, Key Laboratory of Reproduction and Genetics in Ningxia, Department of Histology and Embryology, Ningxia Medical University, Yinchuan 750004, China

Department of Human Anatomy, Inner Mongolia Medical University, Hohhot 010010, China

Received 17 June 2015; Revised 23 August 2015; Accepted 30 August 2015

Academic Editor: Mohammed Rachidi

Copyright © 2015 Yanzhou Yang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Ovarian follicular damages were caused by cryoinjury during the process of ovarian vitrification and ischemia-reperfusion during the process of ovarian transplantation. And appropriate FSH plays an important role in antiapoptosis during ovarian follicle development. Therefore, in this study, 0.3 IU-mL FSH was administered into medium during mouse ovarian cryopreservation by vitrification to ascertain the function of FSH on ovarian vitrification and avascular transplantation. The results suggested that the expressions of Cx37, Cx43, apoptotic molecular caspase-3, and angiogenesis molecular VEGF were confirmed using immunohistochemistry, western blotting, and real-time PCR, and the results suggested that the treatment with FSH remarkably increased the number of morphologically normal follicles in vitrified-warmed ovaries by upregulating the expression of Cx37, Cx43, VEGF, and VEGF receptor 2, but downregulating the expression of caspase-3. In addition, the vitrified-warmed ovaries were transplanted, and the related fertility was analyzed, and the results suggested that the fertility, neoangiogenesis, and follicle reserve were remarkably increased in the FSH administrated group. Taken together, administration of 0.3 IU-mL FSH during ovarian cryopreservation by vitrification can maintain ovarian survival during ovarian vitrification and increases the blood supply with avascular transplantation via upregulation of Cx43, Cx37, and VEGF-VEGFR2, as well as through its antiapoptotic effects.

Author: Yanzhou Yang, Jie Chen, Hao Wu, Xiuying Pei, Qing Chang, Wenzhi Ma, Huiming Ma, Changchun Hei, Xiaomin Zheng, Yufang Cai,



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