Antineoplastic Activities of MT81 and Its Structural Analogue in Ehrlich Ascites Carcinoma-Bearing Swiss Albino MiceReport as inadecuate




Antineoplastic Activities of MT81 and Its Structural Analogue in Ehrlich Ascites Carcinoma-Bearing Swiss Albino Mice - Download this document for free, or read online. Document in PDF available to download.

Oxidative Medicine and Cellular Longevity - Volume 3 2010, Issue 1, Pages 61-70



Department of Human Physiology with Community Health, Vidyasagar University, Midnapore, West Bengal, India

Department of Pharmaceutical Technology, Jadavpur University, Kolkata, West Bengal, India

Received 3 October 2009; Revised 31 October 2009; Accepted 2 November 2009

Copyright © 2010 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Many fungal toxins exhibit in vitro and in vivo antineoplastic effects on various cancer cell types. Luteoskyrin, a hydroxyanthraquinone has been proved to be a potent inhibitor against Ehrlich ascites tumor cells. The comparative antitumor activity and antioxidant status of MT81 and its structural analogue Acetic acid-MT81 Aa-MT81 having polyhydroxyanthraquinone structure were assessed against Ehrlich ascites carcinoma EAC tumor in mice. The in vitro cytotoxicity was measured by the viability of EAC cells after direct treatment of the said compounds. In in vivo study, MT81 and its structural analogue were administered i.p. at the two different doses 5, 7 mg MT81; 8.93, 11.48 mg Aa-MT81-kg body weight for 7 days after 24 hrs. of tumor inoculation. The activities were assessed using mean survival time MST, increased life span ILS, tumor volume, viable tumor cell count, peritoneal cell count, protein percentage and hematological parameters. Antioxidant status was determined by malondialdehyde MDA and reduced glutathione GSH content, and by the activity of superoxide dismutase SOD and catalase CA T. MT81 and its structural analogues increased the mean survival time, normal peritoneal cell count. They decreased the tumor volume, viable tumor cell count, hemoglobin percentage and packed cell volume. Differential counts of WBC, total counts of RBC and WBC that altered by EAC inoculation, were restored in a dose-dependent manner. Increased MDA and decreased GSH content and reduced activity of SOD, and catalase in EAC bearing mice were returned towards normal after the treatment of MT81 and its structural analogue. Being less toxic than parent toxin MT81, the structural analogue showed more prominent antineoplastic activities against EAC cells compared to MT81. At the same time, both compounds exhibit to some extent antioxidant potential for the EAC-bearing mice.





Author: Sujata Maiti Choudhury, Malaya Gupta, and Upal Kanti Majumder

Source: https://www.hindawi.com/



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