Determination of Serological Differentiation Index Values of Turnip Yellow Mosaic Virus TYMV Strains by Means of Rocket ImmunoelectrophoresisReport as inadecuate




Determination of Serological Differentiation Index Values of Turnip Yellow Mosaic Virus TYMV Strains by Means of Rocket Immunoelectrophoresis - Download this document for free, or read online. Document in PDF available to download.

Acta Botanica Croatica, Vol.49 No.1 December 1990. -

An attempt was made to apply rocket immunoelectro- phoresis RIE technique for the determination of serological differentiation index SDI values between three British strains of turnip yellow mosaic virus TYMV based on the height of »rocket« precipitates. The experiments were conducted in l°-o agar gel containing antiserum to Edinburgh E strain of the virus, the holes being charged separately with purified E and two other strains. Following experiments with E strain in several subsequent twofold dilutions, a calibration graph showing linear proportionality was drawn in a coordinate system determined by steps of virus dilutions and logarithm of »rockets« heights. By interpolation procedure SDIs of two heterologous strains against E were found fairly comparable with SDI values obtained by double radial immunodiffusion technique.



Author: Mladen Krajačić - ; Hrvatska Đorđe Mamula - ; Hrvatska

Source: http://hrcak.srce.hr/



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