Evaluation of Lentiviral-Mediated Expression of Sodium Iodide Symporter in Anaplastic Thyroid Cancer and the Efficacy of In Vivo Imaging and TherapyReport as inadecuate




Evaluation of Lentiviral-Mediated Expression of Sodium Iodide Symporter in Anaplastic Thyroid Cancer and the Efficacy of In Vivo Imaging and Therapy - Download this document for free, or read online. Document in PDF available to download.

Journal of OncologyVolume 2011 2011, Article ID 178967, 7 pages

Research Article

Institute of Clinical Medicine, National Yang-Ming University, Taipei 11221, Taiwan

Molecular and Genetic Imaging Core, NRPGM, Taipei 11221, Taiwan

Department of Medical Imaging and Radiological Sciences, Kaohsiung Medical University, Kaohsiung 80708, Taiwan

Faculty of Medicine, National Yang-Ming University, Taipei 11221, Taiwan

Department of Biotechnology, TransWorld University, Yunlin 64063, Taiwan

National PET-Cyclotron Center, Taipei Veterans General Hospital, Taipei 11221, Taiwan

Department of Orthopaedics & Traumatology, Taipei Veterans General Hospital, Taipei 11221, Taiwan

Stem Cell Research Center, National Yang-Ming University, Taipei 11221, Taiwan

Institute of Pharmacology, National Yang-Ming University, Taipei 11221, Taiwan

Division of General Surgery, Department of Surgery, Taipei Veterans General Hospital, Taipei 11221, Taiwan

Department of Biomedical Imaging and Radiological Sciences, National Yang-Ming University, Taipei 11221, Taiwan

Department of Nuclear Medicine, National Yang-Ming University Medical School, Taipei 11221, Taiwan

Received 1 July 2011; Revised 3 September 2011; Accepted 22 September 2011

Academic Editor: Volker Rudat

Copyright © 2011 Chien-Chih Ke et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Anaplastic thyroid carcinoma ATC is one of the most deadly cancers. With intensive multimodalities of treatment, the survival remains low. ATC is not sensitive to

I therapy due to loss of sodium iodide symporter NIS gene expression. We have previously generated a stable human NIS-expressing ATC cell line, ARO, and the ability of iodide accumulation was restored. To make NIS-mediated gene therapy more applicable, this study aimed to establish a lentiviral system for transferring hNIS gene to cells and to evaluate the efficacy of in vitro and in vivo radioiodide accumulation for imaging and therapy. Lentivirus containing hNIS cDNA were produced to transduce ARO cells which do not concentrate iodide. Gene expression, cell function, radioiodide imaging and treatment were evaluated in vitro and in vivo. Results showed that the transduced cells were restored to express hNIS and accumulated higher amount of radioiodide than parental cells. Therapeutic dose of

I effectively inhibited the tumor growth derived from transduced cells as compared to saline-treated mice. Our results suggest that the lentiviral system efficiently transferred and expressed hNIS gene in ATC cells. The transduced cells showed a promising result of tumor imaging and therapy.





Author: Chien-Chih Ke, Ya-Ju Hsieh, Luen Hwu, Fu-Hui Wang, Fu-Du Chen, Lee-Shing Chu, Oscar K. Lee, Chin-Wen Chi, Chen-Hsen Lee, 

Source: https://www.hindawi.com/



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