Prospection and Evaluation of Hemi Cellulolytic Enzymes Using Untreated and Pretreated Biomasses in Two Argentinean Native TermitesReport as inadecuate




Prospection and Evaluation of Hemi Cellulolytic Enzymes Using Untreated and Pretreated Biomasses in Two Argentinean Native Termites - Download this document for free, or read online. Document in PDF available to download.

Saccharum officinarum bagasse common name: sugarcane bagasse and Pennisetum purpureum also known as Napier grass are among the most promising feedstocks for bioethanol production in Argentina and Brazil. In this study, both biomasses were assessed before and after acid pretreatment and following hydrolysis with Nasutitermes aquilinus and Cortaritermes fulviceps termite gut digestome. The chemical composition analysis of the biomasses after diluted acid pretreatment showed that the hemicellulose fraction was partially removed. The hemi cellulolytic activities were evaluated in bacterial culture supernatants of termite gut homogenates grown in treated and untreated biomasses. In all cases, we detected significantly higher endoglucanase and xylanase activities using pretreated biomasses compared to untreated biomasses, carboxymethylcellulose and xylan. Several protein bands with hemi cellulolytic activity were detected in zymograms and two-dimensional gel electrophoresis. Some proteins of these bands or spots were identified as xylanolytic peptides by mass spectrometry. Finally, the diversity of cultured cellulolytic bacterial endosymbionts associated to both Argentinean native termite species was analyzed. This study describes, for the first time, bacterial endosymbionts and endogenous hemi cellulases of two Argentinean native termites as well as their potential application in degradation of lignocellulosic biomass for bioethanol production.



Author: Emiliano Ben Guerrero, Joel Arneodo, Raquel Bombarda Campanha, Patrícia Abrão de Oliveira, Mônica T. Veneziano Labate, Thaís

Source: http://plos.srce.hr/



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