TODRA, a lncRNA at the RAD51 Locus, Is Oppositely Regulated to RAD51, and Enhances RAD51-Dependent DSB Double Strand Break RepairReport as inadecuate




TODRA, a lncRNA at the RAD51 Locus, Is Oppositely Regulated to RAD51, and Enhances RAD51-Dependent DSB Double Strand Break Repair - Download this document for free, or read online. Document in PDF available to download.

Expression of RAD51, a crucial player in homologous recombination HR and DNA double-strand break DSB repair, is dysregulated in human tumors, and can contribute to genomic instability and tumor progression. To further understand RAD51 regulation we functionally characterized a long non-coding lnc RNA, dubbed TODRA Transcribed in the Opposite Direction of RAD51, transcribed 69bp upstream to RAD51, in the opposite direction. We demonstrate that TODRA is an expressed transcript and that the RAD51 promoter region is bidirectional, supporting TODRA expression 7-fold higher than RAD51 in this assay, p = 0.003. TODRA overexpression in HeLa cells induced expression of TPIP, a member of the TPTE family which includes PTEN. Similar to PTEN, we found that TPIP co-activates E2F1 induction of RAD51. Analysis of E2F1-s effect on the bidirectional promoter showed that E2F1 binding to the same site that promotes RAD51 expression, results in downregulation of TODRA. Moreover, TODRA overexpression induces HR in a RAD51-dependent DSB repair assay, and increases formation of DNA damage-induced RAD51-positive foci. Importantly, gene expression in breast tumors supports our finding that E2F1 oppositely regulates RAD51 and TODRA: increased RAD51 expression, which is associated with an aggressive tumor phenotype e.g. negative correlation with positive ER r = -0.22, p = 0.02 and positive PR status r = -0.27, p<0.001; positive correlation with ki67 status r = 0.36, p = 0.005 and HER2 amplification r = 0.41, p = 0.001, correlates as expected with lower TODRA and higher E2F1 expression. However, although E2F1 induction resulted in TPIP downregulation in cell lines, we find that TPIP expression in tumors is not reduced despite higher E2F1 expression, perhaps contributing to increased RAD51 expression. Our results identify TPIP as a novel E2F1 co-activator, suggest a similar role for other TPTEs, and indicate that the TODRA lncRNA affects RAD51 dysregulation and RAD51-dependent DSB repair in malignancy. Importantly, gene expression in breast tumors supports our finding that E2F1 oppositely regulates RAD51 and TODRA: increased RAD51 expression, which is associated with an aggressive tumor phenotype e.g. negative correlation with positive ER r = -0.22, p = 0.02 and positive PR status r = -0.27, p<0.001; positive correlation with ki67 status r = 0.36, p = 0.005 and HER2 amplification r = 0.41, p = 0.001, correlates as expected with lower TODRA and higher E2F1 expression. However, although E2F1 induction resulted in TPIP downregulation in cell lines, we find that TPIP expression in tumors is not reduced despite higher E2F1 expression, perhaps contributing to increased RAD51 expression. Our results identify TPIP as a novel E2F1 co-activator, suggest a similar role for other TPTEs, and indicate that the TODRA lncRNA affects RAD51 dysregulation and RAD51-dependent DSB repair in malignancy.



Author: Inbal Gazy , David A. Zeevi , Paul Renbaum, Sharon Zeligson, Lital Eini, Dana Bashari, Yoav Smith, Amnon Lahad, Michal Goldberg,

Source: http://plos.srce.hr/



DOWNLOAD PDF




Related documents