Improved Recovery of Exfoliated Colonocytes from Feces Using Newly Developed Immunomagnetic BeadsReport as inadecuate




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Gastroenterology Research and PracticeVolume 2008 2008, Article ID 605273, 7 pages

Research Article

Investigative Treatment Division, Research Center for Innovative Oncology, National Cancer Center Hospital East, 6-5-1 Kashiwanoha, Kashiwa 277-8577, Japan

Department of Gastroenterological Surgery, Graduate School of Medical Sciences, Kumamoto University, 1-1-1 Honjo, Kumamoto 860-8556, Japan

Tsukuba Research Laboratories, JSR Corporation, 25 Miyuki-gaoka, Tsukuba 305-0841, Japan

Department of Surgery, National Cancer Center Hospital, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045, Japan

Received 11 September 2008; Accepted 7 November 2008

Academic Editor: Yoshio Yamaoka

Copyright © 2008 Yoshikatsu Koga et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

We demonstrated the feasibility of a new methodology for isolating colonocytes from feces. To reduce costs and improve the recovery rate of colonocytes from feces, we attempted to develop new immunomagnetic beads. Several sizes of magnetic beads were prepared and tagged with a monoclonal antibody against EpCAM. We made several new monoclonal antibodies against EpCAM, and each monoclonal antibody was tagged to the magnetic beads. In the simulation, the most efficient recovery of HT-29 cells was obtained using the smallest size of beads. Also, beads tagged with a monoclonal antibody with a higher affinity against EpCAM had a higher recovery rate. Similar results were obtained when the smallest size of beads with the highest-affinity monoclonal antibody was applied to clinical samples. The newly developed immunomagnetic beads may be useful for isolating colorectal cancer cells from feces, enabling the cytological or molecular biological diagnosis of CRC.





Author: Yoshikatsu Koga, Masahiro Yasunaga, Satoshi Katayose, Yoshihiro Moriya, Takayuki Akasu, Shin Fujita, Seiichiro Yamamoto, Hi

Source: https://www.hindawi.com/



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