Petasites japonicus Stimulates the Proliferation of Mouse Spermatogonial Stem CellsReport as inadecuate




Petasites japonicus Stimulates the Proliferation of Mouse Spermatogonial Stem Cells - Download this document for free, or read online. Document in PDF available to download.

Oriental natural plants have been used as medical herbs for the treatment of various diseases for over 2,000 years. In this study, we evaluated the effect of several natural plants on the preservation of male fertility by assessing the ability of plant extracts to stimulate spermatogonial stem cell SSC proliferation by using a serum-free culture method. In vitro assays showed that Petasites japonicus extracts, especially the butanol fraction, have a significant effect on germ cells proliferation including SSCs. The activity of SSCs cultured in the presence of the Petasites japonicus butanol fraction was confirmed by normal colony formation and spermatogenesis following germ cell transplantation of the treated SSCs. Our findings could lead to the discovery of novel factors that activate SSCs and could be useful for the development of technologies for the prevention of male infertility.



Author: Hye-Ryun Kang , Yong-An Lee , Yong-Hee Kim, Dong Gu Lee, Bang-Jin Kim, Ki-Jung Kim, Byung-Gak Kim, Myeong-Geun Oh, Chan Kyu Han,

Source: http://plos.srce.hr/



DOWNLOAD PDF




Related documents