Effects of Awakening and the Use of Topical Dexamethasone and Levofloxacin on the Cytokine Levels in Tears Following Corneal TransplantationReport as inadecuate




Effects of Awakening and the Use of Topical Dexamethasone and Levofloxacin on the Cytokine Levels in Tears Following Corneal Transplantation - Download this document for free, or read online. Document in PDF available to download.

Journal of Immunology Research - Volume 2014 2014, Article ID 570685, 8 pages -

Clinical Study

Department of Ophthalmology, University of Debrecen Clinical Centre, Nagyerdei Krt. 98, Debrecen 4012, Hungary

Department of Ophthalmology, Faculty of Medicine, University of Szeged, Korányi fasor 10-11, Szeged 6720, Hungary

Stem Cells and Eye Research Laboratory, Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Debrecen, Nagyerdei Krt. 98, Debrecen 4012, Hungary

Department of Immunology, University of Debrecen Clinical Centre, Nagyerdei Krt. 98, Debrecen 4012, Hungary

Received 24 March 2014; Revised 28 August 2014; Accepted 11 September 2014; Published 12 October 2014

Academic Editor: Kai Kaarniranta

Copyright © 2014 Mariann Fodor et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Objectives. To study the short-term effect of eye opening and use of topical dexamethasone phosphate 0.1% and levofloxacin 0.5% on the cytokine levels in human tears. Methods. Prospective experimental design was used for tear collection from eyes of 10 healthy controls and 20 patients four days after penetrating keratoplasty PKP at awakening and after instilling dexamethasone or levofloxacin. The concentrations of different cytokines were measured by cytometric bead array. Results. At eye opening, IL-6 levels were higher in the PKP group as compared to the controls. Thirty minutes later, the released levels of IL-10, IL-13, IL-17, IFNγ, and CCL5 increased in controls, while CXCL8 decreased in both control and PKP groups. The release of the cytokines remained stable after 30 mins except for IFNγ, which showed a decrease in the controls following levofloxacin instillation. No short-term effects of the topically used dexamethasone and levofloxacin could be detected on the cytokine levels in controls and after PKP. Conclusions. Evidence of changes in the levels and time course of tear cytokines after awakening or eye opening could be established and the short-term confounding effects of dexamethasone and levofloxacin on the levels of released cytokines in human tears could be excluded.





Author: Mariann Fodor, Goran Petrovski, Dorottya Pásztor, Péter Gogolák, Éva Rajnavölgyi, and András Berta

Source: https://www.hindawi.com/



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