The Impact of Various Reactive Oxygen Species on the Formation of Neutrophil Extracellular TrapsReport as inadecuate




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Mediators of InflammationVolume 2012 2012, Article ID 849136, 10 pages

Research Article

Institute for Medical Microbiology and Hygiene, University of Lübeck, Ratzeburger Allee 160, 23538 Lübeck, Germany

Institute of Anatomy, University of Lübeck, Ratzeburger Allee 160, 23538 Lübeck, Germany

Received 4 November 2011; Revised 21 December 2011; Accepted 22 December 2011

Academic Editor: Johan Bylund

Copyright © 2012 Tina Kirchner et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The formation of neutrophil extracellular traps NETs depends on the generation of reactive oxygen species ROS. Previous studies revealed that both NADPH oxidase and myeloperoxidase MPO are required for NET release. However, the contribution of various ROS as well as the role of mitochondria-derived ROS has not been addressed so far. In the present study we aimed to investigate in a systematic and comprehensive manner the contribution of various ROS and ROS-generating pathways to the PMA-induced NET release. By using specific inhibitors, the role of both NADPH oxidase- and mitochondria-derived ROS as well as the contribution of superoxide dismutase SOD and MPO on the NET release was assessed. We could demonstrate that NADPH oxidase function is crucial for the formation of NETs. In addition, we could clearly show the involvement of MPO-derived ROS in NET release. Our results, however, did not provide evidence for the role of SOD- or mitochondria-derived ROS in NET formation.





Author: Tina Kirchner, Sonja Möller, Matthias Klinger, Werner Solbach, Tamás Laskay, and Martina Behnen

Source: https://www.hindawi.com/



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