Enhanced Critical Size Defect Repair in Rabbit Mandible by Electrospun Gelatin-β-TCP Composite Nanofibrous MembranesReport as inadecuate




Enhanced Critical Size Defect Repair in Rabbit Mandible by Electrospun Gelatin-β-TCP Composite Nanofibrous Membranes - Download this document for free, or read online. Document in PDF available to download.

Journal of Nanomaterials - Volume 2015 2015, Article ID 396916, 9 pages -

Research Article

Department of Geriatric Dentistry, Peking University School and Hospital of Stomatology, Beijing 100081, China

Department of Dental Materials, Peking University School and Hospital of Stomatology, Beijing 100081, China

Department of Orthodontics, Peking University School and Hospital of Stomatology, Beijing 100081, China

National Engineering Laboratory for Digital and Material Technology of Stomatology, Beijing 100081, China

Beijing Laboratory of Biomedical Materials, Peking University School and Hospital of Stomatology, Beijing 100081, China

Received 10 October 2014; Accepted 15 December 2014

Academic Editor: Krasimir Vasilev

Copyright © 2015 Mingming Xu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The design and fabrication of biodegradable barrier membranes with satisfactory structure and composition remain a considerable challenge for periodontal tissue regeneration. We have developed a biomimetic nanofibrous membrane made from a composite of gelatin and β-tricalcium phosphate β-TCP. We previously confirmed the in vitro biological performance of the membrane material, but the efficacy of the membranes in promoting bone repair in situ has not yet been examined. Gelatin-β-TCP composite nanofibers were fabricated by incorporation of 20 wt.% β-TCP nanoparticles into electrospun gelatin nanofibers. Electron microscopy showed that the composite membranes presented a nonwoven structure with an interconnected porous network and had a rough surface due to the β-TCP nanoparticles, which were distributed widely and uniformly throughout the gelatin-fiber matrix. The repair efficacy of rabbit mandible defects implanted with bone substitute Bio-Oss and covered with the gelatin-β-TCP composite nanofibrous membrane was evaluated in comparison with pure gelatin nanofibrous membrane. Gross observation, histological examination, and immunohistochemical analysis showed that new bone formation and defect closure were significantly enhanced by the composite membranes compared to the pure gelatin ones. From these results, we conclude that nanofibrous gelatin-β-TCP composite membranes could serve as effective barrier membranes for guided tissue regeneration.





Author: Mingming Xu, Xuehui Zhang, Song Meng, Xiaohan Dai, Bing Han, and Xuliang Deng

Source: https://www.hindawi.com/



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