Decreased MicroRNA-125a-3p Contributes to Upregulation of p38 MAPK in Rat Trigeminal Ganglions with Orofacial Inflammatory PainReport as inadecuate




Decreased MicroRNA-125a-3p Contributes to Upregulation of p38 MAPK in Rat Trigeminal Ganglions with Orofacial Inflammatory Pain - Download this document for free, or read online. Document in PDF available to download.

Orofacial inflammatory pain is a difficult clinical problem, and the specific molecular mechanisms for this pain remain largely unexplained. The present study aimed to determine the differential expression of microRNAs miRNAs and disclose the underlying role of miR-125a-3p in orofacial inflammatory pain induced by complete Freund-s adjuvant CFA. Thirty-two differentially expressed miRNAs were first screened using a microarray chip in ipsilateral trigeminal ganglions TGs following CFA injection into the orofacial skin innervated by trigeminal nerve, and a portion of them, including miR-23a* -24-2* -26a -92a -125a-3p -183 and -299 were subsequently selected and validated by qPCR. The target genes were predicted based on the miRWalk website and were further analyzed by gene ontology GO. Further studies revealed miR-125a-3p expression was down-regulated, whereas both the expression of p38 MAPK mitogen-activated protein kinase alpha and CGRP calcitonin gene-related peptide were up-regulated in ipsilateral TGs at different time points after CFA injection compared with control. Furthermore, mechanistic study revealed that miR-125a-3p negatively regulates p38 alpha gene expression and is positively correlated with the head withdrawal threshold reflecting pain. Luciferase assay showed that binding of miR-125a-3p to the 3′UTR of p38 alpha gene suppressed the transcriptional activity, and overexpression of miR-125a-3p significantly inhibited the p38 alpha mRNA level in ND8-34 cells. Taken together, our results show that miR-125a-3p is negatively correlated with the development and maintenance of orofacial inflammatory pain via regulating p38 MAPK.



Author: Yingchun Dong , Pengfei Li, Yanhong Ni, Junjie Zhao, Zhiqiang Liu

Source: http://plos.srce.hr/



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