A comparative study reveals the higher resolution of RAPD over ARDRA for analyzing diversity of Nostoc strainsReport as inadecuate




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3 Biotech

, 7:125

First Online: 01 June 2017Received: 01 November 2016Accepted: 06 March 2017DOI: 10.1007-s13205-017-0779-5

Cite this article as: Chakdar, H. & Pabbi, S. 3 Biotech 2017 7: 125. doi:10.1007-s13205-017-0779-5

Abstract

Nostoc is a diverse genus of filamentous cyanobacteria with tremendous potential for agricultural and industrial applications. Morphometric methods and routine 16S rDNA-based identification undermines the genetic diversity and impedes strain-level differentiation. A comparative study to deduce the discriminatory power of random amplified polymorphic DNA RAPD and amplified ribosomal DNA restriction analysis ARDRA for analyzing the genetic diversity of 20 Nostoc strains of diverse geographical origin was carried out. The RAPD primer used in the study generated 100% polymorphic profile. HIP TG primer produced the highest number of bands and fragments. Five primers, viz. OPA 08, OPA 11, HIP GC, OPAH 02 and OPF 05 could produce unique bands for 11 strains. Cluster analysis using the RAPD profile showed 12.5–25% similarity among the strains. Following in silico restriction analysis, two restriction enzymes, viz. HaeIII and HinfI were selected for ARDRA. However, clustering based on the restriction pattern showed 22.5–100% similarity. Results of the present study clearly indicate higher resolution of RAPD which can be reliably used for strain-level differentiation of Nostoc strains.

KeywordsNostoc RAPD ARDRA Genetic diversity Discriminatory power 



Author: Hillol Chakdar - Sunil Pabbi

Source: https://link.springer.com/







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