Amniotic Membrane-Derived Mesenchymal Cells and Their Conditioned Media: Potential Candidates for Uterine Regenerative Therapy in the HorseReport as inadecuate




Amniotic Membrane-Derived Mesenchymal Cells and Their Conditioned Media: Potential Candidates for Uterine Regenerative Therapy in the Horse - Download this document for free, or read online. Document in PDF available to download.

Amniotic membrane-derived mesenchymal cells AMCs are considered suitable candidates for a variety of cell-based applications. In view of cell therapy application in uterine pathologies, we studied AMCs in comparison to cells isolated from the endometrium of mares at diestrus EDCs being the endometrium during diestrus and early pregnancy similar from a hormonal standpoint. In particular, we demonstrated that amnion tissue fragments AM shares the same transcriptional profile with endometrial tissue fragments ED, expressing genes involved in early pregnancy AbdB-like Hoxa genes, pre-implantantion conceptus development Erα, PR, PGRMC1 and mPR and their regulators Wnt7a, Wnt4a. Soon after the isolation, only AMCs express Wnt4a and Wnt7a. Interestingly, the expression levels of prostaglandin-endoperoxide synthase 2 PTGS2 were found greater in AM and AMCs than their endometrial counterparts thus confirming the role of AMCs as mediators of inflammation. The expression of nuclear progesterone receptor PR, membrane-bound intracellular progesterone receptor component 1 PGRMC1 and membrane-bound intracellular progesterone receptor mPR, known to lead to improved endometrial receptivity, was maintained in AMCs over 5 passages in vitro when the media was supplemented with progesterone. To further explore the potential of AMCs in endometrial regeneration, their capacity to support resident cell proliferation was assessed by co-culturing them with EDCs in a transwell system or culturing in the presence of AMC-conditioned medium AMC-CM. A significant increase in EDC proliferation rate exhibited the crucial role of soluble factors as mediators of stem cells action. The present investigation revealed that AMCs, as well as their derived conditioned media, have the potential to improve endometrial cell replenishment when low proliferation is associated to pregnancy failure. These findings make AMCs suitable candidates for the treatment of endometrosis in mares.



Author: Bruna Corradetti, Alessio Correani, Alessio Romaldini, Maria Giovanna Marini, Davide Bizzaro, Claudia Perrini, Fausto Cremonesi ,

Source: http://plos.srce.hr/



DOWNLOAD PDF




Related documents