From iPSC towards cardiac tissue—a road under constructionReport as inadecuate




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Pflügers Archiv - European Journal of Physiology

pp 1–11

Invited Review

Abstract

The possibility to generate induced pluripotent stem cells iPSC opens the way to generate virtually all cell types of our human body. In combination with modern gene editing techniques like CRISPR-CAS, a new set of powerful tools becomes available for life science. Scientific fields like genotype and cell type-specific pharmacology, disease modeling, stem cell biology, and developmental biology have been dramatically fostered and their faces have been changed. However, as golden as the age of iPSC-derived cells and their manipulation has started, the shine begins to tarnish. Researchers face more and more practical problems intrinsic to the system. These problems are related to the specific culturing conditions which are not yet sufficient to mimic the natural environment of native stem cells differentiating towards adult cells. However, researchers work hard to uncover these factors. Here, we review a common standard approach to generate iPSCs and transduce these to iPSC cardiomyocytes. Further, we review recent achievements and discuss their current limitations and future perspectives. We are on track, but the road is still under construction.

KeywordsCardiac differentiation Induced pluripotent stem cell Myocyte physiology Signaling pathway Long QT syndrome AbbrevationscTnTCardiac troponin-T

EOMESEomesodermin

FGF-2Fibroblast growth factor-2

hESCHuman embryonal stem cells

KChIPK-channel interacting proteins

Mesp1Mesoderm posterior basic helix-loop-helix transcription factor 1

Oct-4Octamer-binding transcription factor 4

PAX-6Paired-box-gene-6

SmadMothers against decapentaplegic homolog

Sox2Sex determining region Y-box 2

TGFβ-1Transforming growth factor beta 1

WntWingless-related integration site





Author: Stefan Peischard - Ilaria Piccini - Nathalie Strutz-Seebohm - Boris Greber - Guiscard Seebohm

Source: https://link.springer.com/







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