Effects of Oxidized Phospholipids on Gene Expression in RAW 264.7 Macrophages: A Microarray StudyReport as inadecuate




Effects of Oxidized Phospholipids on Gene Expression in RAW 264.7 Macrophages: A Microarray Study - Download this document for free, or read online. Document in PDF available to download.

Oxidized phospholipids oxPLs are components of oxidized LDL oxLDL. It is known that oxLDL activates expression of a series of atherogenic genes and their oxPLs contribute to their biological activities. In this study we present the effects of 1-palmitoyl-2-glutaroyl-sn-glycero-3-phosphocholine PGPC and 1-palmitoyl-2-5-oxovaleroyl-sn-glycero-3-phosphocholine POVPC on gene expression in RAW 264.7 macrophages using cDNA microarrays. PGPC affected the regulation of 146 genes, whereas POVPC showed only very minor effects. PGPC preferentially influenced expression of genes related to cell death, angiogenesis, cholesterol efflux, procoagulant mechanisms, atherogenesis, inflammation, and cell cycle. Many of these effects are known from studies with oxLDL or oxidized 1-hexadecanoyl-2-eicosatetra-5′,8′,11′,14′-enoyl-sn-glycero-3-phosphocholine oxPAPC, containing PGPC in addition to other oxPL species. It is known that POVPC efficiently reacts with proteins by Schiff base formation, whereas PGPC only physically interacts with its biological targets. POVPC seems to affect cell physiology to a great extent on the protein level, whereas PGPC gives rise to both the modulation of protein function and regulation on the transcriptional level.



Author: Daniel Koller, Hubert Hackl, Juliane Gertrude Bogner-Strauß, Albin Hermetter

Source: http://plos.srce.hr/



DOWNLOAD PDF




Related documents