Time to Seroconversion in HIV-Exposed Subjects Carrying Protective versus Non Protective KIR3DS1-L1 and HLA-B GenotypesReport as inadecuate




Time to Seroconversion in HIV-Exposed Subjects Carrying Protective versus Non Protective KIR3DS1-L1 and HLA-B Genotypes - Download this document for free, or read online. Document in PDF available to download.

Natural killer NK cells play a role in the clearance of viral infections. Combinations of alleles at the polymorphic HLA-B locus and the NK cell surface killer immunoglobulin-like receptor locus KIR3DL1-S1 have been shown to influence time to AIDS in HIV-infected individuals and risk of seroconversion in HIV exposed seronegative HESN subjects. Here, we assessed time to seroconversion or duration of seronegative status in a group of 168 HIV exposed individuals, including 74 seroconverters and 94 HESN based on carriage or not of KIR3DL1-S1-HLA-B genotypes previously shown to be associated with protection from infection and-or slow time to AIDS. KIR3DL1-S1 genotyping was performed by sequence-specific primer polymerase chain reaction using two pairs of specific primers for each locus. The MHC class IB locus was typed to four-position resolution to resolve Bw4 and Bw6 alleles and the amino acid present at position 80. KIR3DL1-S1 heterozygotes became HIV infected significantly faster than KIR3DS1 homozygotes. Individuals who carried both KIR3DS1 and Bw4*80I did not remain HIV seronegative longer than those from a control group who were homozygous for HLA-Bw6 and carried no HLA-A locus Bw4 alleles Subjects who were *h-*y+B*57 showed a trend towards slower time to serconversion than those with other KIR3DL1 homozygous and KIR3DL1-S1 heterozygous genotypes. Thus, KIR3DS1 homozygosity is associated with protection from HIV infection while co-carriage of KIR3DS1 and Bw4*80I is not. The requirements for protection from HIV infection can differ from those that influence time to AIDS in HIV infected individuals.



Author: Benjamin J. M. Tallon, Julie Bruneau, Christos M. Tsoukas, Jean-Pierre Routy, Zahra Kiani, Xianming Tan, Nicole F. Bernard

Source: http://plos.srce.hr/



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