The Suppressive Effect of Resveratrol on HIF-1α and VEGF Expression after Warm Ischemia and Reperfusion in Rat LiverReport as inadecuate




The Suppressive Effect of Resveratrol on HIF-1α and VEGF Expression after Warm Ischemia and Reperfusion in Rat Liver - Download this document for free, or read online. Document in PDF available to download.

Background

Hypoxia-inducible factor-1α HIF-1α is overexpressed in many human tumors and their metastases, and is closely associated with a more aggressive tumor phenotype. The aim of the present study was to investigate the effect of resveratrol RES on the expression of ischemic-induced HIF-1α and vascular endothelial growth factor VEGF in rat liver.

Methods

Twenty-four rats were randomized into Sham, ischemia-reperfusion I-R, and RES preconditioning groups. I-R was induced by portal pedicle clamping for 60 minutes followed by reperfusion for 60 minutes. The rats in RES group underwent the same surgical procedure as I-R group, and received 20 mg-kg resveratrol intravenously 30 min prior to ischemia. Blood and liver tissue samples were collected and subjected to biochemical assays, RT-PCR, and Western blot assays.

Results

I-R resulted in a significant P<0.05 increase in liver HIF-1α and VEGF at both mRNA and protein levels 60 minutes after reperfusion. The mRNA and protein expressions of HIF-1α and VEGF decreased significantly in RES group when compared to I-R group P<0.05.

Conclusion

The inhibiting effect of RES on the expressions of HIF-1α and VEGF induced by I-R in rat liver suggested that HIF-1α-VEGF could be a promising drug target for RES in the development of an effective anticancer therapy for the prevention of hepatic tumor growth and metastasis.



Author: Mei Zhang , Wujun Li , Liang Yu, Shengli Wu

Source: http://plos.srce.hr/



DOWNLOAD PDF




Related documents