Expression and functional analyses of a Kinesin gene GhKIS13A1 from cotton Gossypium hirsutum fiberReport as inadecuate




Expression and functional analyses of a Kinesin gene GhKIS13A1 from cotton Gossypium hirsutum fiber - Download this document for free, or read online. Document in PDF available to download.

BMC Biotechnology

, 17:50

Plant biotechnology

Abstract

BackgroundCotton fiber, a natural fiber widely used in the textile industry, is differentiated from single cell of ovule epidermis. A large number of genes are believed to be involved in fiber formation, but so far only a few fiber genes have been isolated and functionally characterized in this developmental process. The Kinesin13 subfamily was found to play key roles during cell division and cell elongation, and was considered to be involved in the regulation of cotton fiber development.

ResultsThe full length of coding sequence of GhKIS13A1 was cloned using cDNA from cotton fiber for functional characterization. Expression pattern analysis showed that GhKIS13A1 maintained a lower expression level during cotton fiber development. Biochemical assay showed that GhKIS13A1 has microtubule binding activity and basal ATPase activity that can be activated significantly by the presence of microtubules. Overexpression of GhKIS13A1 in Arabidopsis reduced leaf trichomes and the percentage of three-branch trichomes, and increased two-branch and shriveled trichomes compared to wild-type. Additionally, the expression of GhKIS13A1 in the Arabidopsis Kinesin-13a-1 mutant rescued the defective trichome branching pattern of the mutant, making its overall trichome branching pattern back to normal.

ConclusionsOur results suggested that GhKIS13A1 is functionally compatible with AtKinesin-13A regarding their role in regulating the number and branching pattern of leaf trichomes. Given the developmental similarities between cotton fibers and Arabidopsis trichomes, it is speculated that GhKIS13A1 may also be involved in the regulation of cotton fiber development.

KeywordsKinesin13 subfamily Cotton fiber Trichome GhKIS13A1 AbbreviationsAMP-PNP5’-adenylylimidodiphosphate

ATPAdenosine triphosphate

BSABovine serum albumin

DPADay post anthesis

GTPGuanosine triphosphate

IPTGIsopropyl thio-β-D-galactoside

MSMurashige and Skoog

MTsMicrotubules

ORFOpen reading frame

PiInorganic phosphate

qPCRReal-time quantative PCR

SDS-PAGESodium dodecyl sulfate-polyacrylamide gel electrophoresis





Author: Yan-Jun Li - Shou-Hong Zhu - Xin-Yu Zhang - Yong-Chang Liu - Fei Xue - Lan-Jie Zhao - Jie Sun

Source: https://link.springer.com/



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