Genetic Diversity of and Differentiation among Five Populations of Blunt Snout Bream Megalobrama amblycephala Revealed by SRAP Markers: Implications for Conservation and ManagementReport as inadecuate




Genetic Diversity of and Differentiation among Five Populations of Blunt Snout Bream Megalobrama amblycephala Revealed by SRAP Markers: Implications for Conservation and Management - Download this document for free, or read online. Document in PDF available to download.

The blunt snout bream Megalobrama amblycephala is an important freshwater aquaculture fish throughout China. Because of widespread introductions of this species to many regions, the genetic diversity of wild and natural populations is now threatened. In the present study, SRAP sequence-related amplified polymorphism markers were used to assess genetic diversity of blunt snout bream. Three natural populations Liangzi Lake, Poyang Lake and Yuni Lake, one cultured population Nanxian and one genetic strain ‘Pujiang No. 1’ of blunt snout bream were screened with 88 SRAP primer combinations, of which 13 primer pairs produced stable and reproducible amplification patterns. In total, 172 bands were produced, of which 132 bands were polymorphic. Nei-s gene diversity h and Shannon-s information index I values provided evidence of differences in genetic diversity among the five populations Poyang Lake>Liangzi Lake>Nanxian>‘Pujiang No. 1’>Yuni Lake. Based on cluster analysis conducted on genetic distance values, the five blunt snout bream populations were divided into three groups, Poyang Lake and Liangzi Lake natural populations, Nanxian and ‘Pujiang No. 1’ cultured population and genetically selected strain, and Yuni Lake natural population. Significant genetic differentiation was found among the five populations using analysis of molecular variance AMOVA, with more genetic divergence existing among populations 55.49%, than within populations 44.51%. This molecular marker technique is a simple and efficient method to quantify genetic diversity within and among fish populations, and is employed here to help manage and conserve germplasm variability of blunt snout bream and to support the ongoing selective breeding programme for this fish.



Author: Wei Ji , Gui-Rong Zhang , Wei Ran, Jonathan P. A. Gardner, Kai-Jian Wei , Wei-Min Wang, Gui-Wei Zou

Source: http://plos.srce.hr/



DOWNLOAD PDF




Related documents