Use of Preservative Agents and Antibiotics for Increased Poliovirus Survival on Positively Charged FiltersReport as inadecuate




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Food and Environmental Virology

pp 1–12

First Online: 14 June 2017Received: 10 April 2017Accepted: 06 June 2017DOI: 10.1007-s12560-017-9306-4

Cite this article as: Fagnant, C.S., Kossik, A.L., Zhou, N.A. et al. Food Environ Virol 2017. doi:10.1007-s12560-017-9306-4

Abstract

Environmental surveillance of poliovirus PV and other non-enveloped viruses can help identify silent circulation and is necessary to certify eradication. The bag-mediated filtration system is an efficient method to filter large volumes of environmental waters at field sites for monitoring the presence of viruses. As filters may require long transit times to off-site laboratories for processing, viral inactivation or overgrowth of bacteria and fungi can interfere with virus detection and quantification Miki and Jacquet in Aquatic Microb Ecol 512:195–208, 2008. To evaluate virus survival over time on ViroCap filters, the filters were seeded with PV type 1 PV1 and-or MS2 and then dosed with preservatives or antibiotics prior to storage and elution. These filters were stored at various temperatures and time periods, and then eluted for PV1 and MS2 recovery quantification. Filters dosed with the preservative combination of 2% sodium benzoate and 0.2% calcium propionate had increased virus survival over time when stored at 25 °C, compared to samples stored at 25 °C with no preservatives. While elution within 24 h of filtration is recommended, if storage or shipping is required then this preservative mixture can help preserve sample integrity. Addition of an antibiotic cocktail containing cephapirin, gentamicin, and Proclin 300 increased recovery after storage at 4 and 25 °C, when compared to storage with no antibiotics. The antibiotic cocktail can aid sample preservation if access to appropriate antibiotics storage is available and sample cold chain is unreliable. This study demonstrated that the use of preservatives or antibiotics is a simple, cost-effective method to improve virus detection from ViroCap cartridge filters over time.

KeywordsViroCap filters Environmental surveillance Poliovirus Filtration Virus recovery Preservatives 



Author: Christine Susan Fagnant - Alexandra Lynn Kossik - Nicolette Angela Zhou - Liliana Sánchez-Gonzalez - Jill Christin Falma

Source: https://link.springer.com/







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