The Intracellular HBV DNAs as Novel and Sensitive Biomarkers for the Clinical Diagnosis of Occult HBV Infection in HBeAg Negative Hepatocellular Carcinoma in ChinaReport as inadecuate




The Intracellular HBV DNAs as Novel and Sensitive Biomarkers for the Clinical Diagnosis of Occult HBV Infection in HBeAg Negative Hepatocellular Carcinoma in China - Download this document for free, or read online. Document in PDF available to download.

This study aimed to investigate the virological status in liver both tumor and adjacent non-tumor tissue, the clinical features and the contribution of occult HBV infection OBI to postoperative prognosis in HBeAg-negative− hepatocellular carcinoma HCC patients in China. Using quantitative TaqMan fluorescent real-time PCR assays, HBV covalently closed circular DNA cccDNA and total DNA tDNA were both quantified in 11 HBsAg− and 57 HBsAg-positive+ pairs of tumor tissue TT and adjacent non-tumor tissue ANTT obtained from HBeAg− HCC patients who received no antiviral treatment and were negative for anti-HCV before surgical treatment. Of 11 HBsAg− patients, 36% were with HBsAb+ HBeAb+ HBcAb+. However, only 9% of the HBsAg− patients were HBsAb− HBeAb+ HBcAb+, which accounted for the majority 93% in the HBsAg+ group. TT and ANTT HBV tDNAs in 11 HCC patients with HBsAg − and HBeAg − were all detectable. HBV cccDNA and tDNA were all lower in the HBsAg− group than those in the HBsAg+ group. By Kaplan-Meier analysis, patients with OBI were associated with a lower risk of cirrhosis and better overall survival OS. The intracellular HBV DNAs, such as HBV cccDNA and tDNA are valuable biological markers for the diagnosis of occult HBV infection in HCC patients. This would assist the clinical implementation of a more personalized therapy for viral re-activation control and improve the survival rate of OBI patients.



Author: Hui Wang, Meng Fang, Xing Gu, Qiang Ji, Dongdi Li, Shu-Qun Cheng, Feng Shen, Chun-Fang Gao

Source: http://plos.srce.hr/



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