Synthesis, Characterization, and Evaluation of a Novel Amphiphilic Polymer RGD-PEG-Chol for Target Drug Delivery SystemReport as inadecuate




Synthesis, Characterization, and Evaluation of a Novel Amphiphilic Polymer RGD-PEG-Chol for Target Drug Delivery System - Download this document for free, or read online. Document in PDF available to download.

The Scientific World Journal - Volume 2014 2014, Article ID 546176, 10 pages -

Research Article

State Key Laboratory of Biotherapy and Department of Pharmacy, West China Hospital, Sichuan University, No. 37 Guoxue Alley, Chengdu, Sichuan 610041, China

State Key Laboratory Breeding Base of Systematic research, Development and Utilization of Chinese Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China

Received 26 August 2013; Accepted 24 October 2013; Published 21 January 2014

Academic Editors: G. Lamberti and S. Mao

Copyright © 2014 Shi Zeng et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

An amphiphilic polymer RGD-PEG-Chol which can be produced in large scale at a very low cost has been synthesized successfully. The synthesized intermediates and final products were characterized and confirmed by

H nuclear magnetic resonance spectrum

H NMR and Fourier transform infrared spectrum FT-IR. The paclitaxel- PTX- loaded liposomes based on RGD-PEG-Chol were then prepared by film formation method. The liposomes had a size within 100 nm and significantly enhanced the cytotoxicity of paclitaxel to B16F10 cell as demonstrated by MTT test IC50 = 0.079 μg-mL of RGD-modified PTX-loaded liposomes compared to 9.57 μg-mL of free PTX. Flow cytometry analysis revealed that the cellular uptake of coumarin encapsulated in the RGD-PEG-Chol modified liposome was increased for HUVEC cells. This work provides a reasonable, facile, and economic approach to prepare peptide-modified liposome materials with controllable performances and the obtained linear RGD-modified PTX-loaded liposomes might be attractive as a drug delivery system.





Author: Shi Zeng, Fengbo Wu, Bo Li, Xiangrong Song, Yu Zheng, Gu He, Cheng Peng, and Wei Huang

Source: https://www.hindawi.com/



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