BdcA, a Protein Important for Escherichia coli Biofilm Dispersal, Is a Short-Chain Dehydrogenase-Reductase that Binds Specifically to NADPHReport as inadecuate




BdcA, a Protein Important for Escherichia coli Biofilm Dispersal, Is a Short-Chain Dehydrogenase-Reductase that Binds Specifically to NADPH - Download this document for free, or read online. Document in PDF available to download.

The Escherichia coli protein BdcA previously referred to as YjgI plays a key role in the dispersal of cells from bacterial biofilms, and its constitutive activation provides an attractive therapeutic target for dismantling these communities. In order to investigate the function of BdcA at a molecular level, we integrated structural and functional studies. Our 2.05 Å structure of BdcA shows that it is a member of the NADPH-dependent short-chain dehydrogenase-reductase SDR superfamily. Structural comparisons with other members of the SDR family suggested that BdcA binds NADPH. This was demonstrated experimentally using thermal denaturation studies, which showed that BcdA binds specifically to NADPH. Subsequent ITC experiments further confirmed this result and reported a Kd of 25.9 µM. Thus, BdcA represents the newest member of the limited number of oxidoreductases shown to be involved in quorum sensing and biofilm dispersal.



Author: Dana M. Lord, Ayse Uzgoren Baran, Thomas K. Wood, Wolfgang Peti, Rebecca Page

Source: http://plos.srce.hr/



DOWNLOAD PDF




Related documents